Each original application for a license to conduct a school shall be accompanied by a license fee equal to ten dollars ($10) for each teacher used or employed at any one time. The first application shall be accompanied by the fee based on the highest number of teachers used or employed at any one time during the twelve months immediately preceding the effective date of this act, or, if the applicant is first commencing to operate after said date, the fee shall be based on the highest number of teachers estimated to be employed at any one time during the first six months of the year for which the license is to be effective. If, during the first six months, such applicant shall use or employ at any one time more teachers than the number he estimated, he shall pay the additional fee due therefor at the time he applies for his next renewal. Each renewal application shall be accompanied by the fee based on the highest number of teachers used or employed at any one time during the license year immediately preceding the year for which the renewal is to be effective. No fee shall be charged for a supplementary application for the approval of additional fields or courses of instruction. Fees for agents shall be five dollars ($5) per year. All license fees shall be paid into the General Fund in the State Treasury through the Department of Revenue. No license fees shall be refunded in the event any license is suspended or revoked.