1 CRR-NY 57.8NY-CRR
1 CRR-NY 57.8
1 CRR-NY 57.8
57.8 Blood tests, environmental sample collection and bird selection for organ culture.
(a) Blood tests.
Blood samples shall be obtained and tested as provided in 9 CFR, part 147, subpart A.
(b) Collection methods for environmental samples.
Environmental samples shall be collected and cultured in accordance with the following procedures or any other procedures approved in writing by the commissioner.
(1) Floor layer operations.
Three samples (two floor litter and one nest-box) shall be collected per house, or per floor if a house has multiple floors.
(i) Floor litter. Two samples shall be collected as follows: four 4 × 4-inch gauze sponges premoistened with double strength skim milk shall be dragged over the floor litter surface for a minimum period of 15 minutes. The gauze sponges used to collect the samples shall be placed in an 18-ounce whirl-pak bag, or 50 ml. Falcon centrifuge tube, two sponges per container with each container containing 5 mls. of double strength skim milk prepared as provided in section 57.9(a) of this Part. This will maintain the moistness of the sample during transport. The container shall be marked with the identity of the type of sample, the house identification, and the floor within the house if there are multiple floors.
(ii) Nest-boxes. One nest-box sample shall be collected by using two sterile 4 × 4-inch gauze sponges premoistened with double strength skim milk prepared as provided in section 57.9(a) of this Part. The two gauze sponges used to collect the sample shall be wiped over the interior surfaces of a minimum of ¼ of the total nest-boxes. The gauze sponges used to collect the sample shall be placed in an 18 ounce whirl-pak bag or 50 ml. Falcon centrifuge tube containing 5 mls. of double strength skim milk prepared as provided in section 57.9(a) of this Part. The container shall be marked with the identity of the type of sample, house identification, and the floor within the house if there are multiple floors.
(2) Caged layer operations.
(i) Manure samples.
(a) Automated manure removal systems. One manure sample shall be collected from each row of cages, using sterile 4 × 4-inch gauze sponges premoistened with double strength skim milk prepared as provided in section 57.9(a) of this Part. Two sponges shall be used for each row. All manure scrapers beneath each tier of cages shall be thoroughly wiped with the sponges.
(b) Nonautomated manure removal systems. (Example: deep-pit housing) Collect the samples by dragging the gauze sponges, premoistened with double strength skim milk, prepared as provided in section 57.9(a) of this Part, along the entire length of the manure pile beneath the cages of each row. The gauze sponges used to collect the samples from the two rows shall be placed in an 18 ounce whirl-pak bag or 50 ml. Falcon centrifuge tube containing 5 mls. of double-strength skim milk prepared as provided in section 57.9(a) of this Part. The container shall be marked with the house identification, and the location of the bank and rows from which the sample was taken.
(ii) Egg transport machinery samples. One egg transport machinery sample shall be collected from each row of cages by wiping at least ½ of the total surface area of the escalator belts at the front of each row of cages with two sterile 4 × 4-inch gauze sponges. The sponges will be premoistened with double strength skim milk prepared as provided in section 57.9(a) of this Part. The gauze sponges used to collect the samples from the two rows of each bank shall be placed in an 18 ounce whirl-pak bag or 50 ml. Falcon centrifuge tube containing 5 mls. of double strength skim milk prepared as provided in section 57.9(a) of this Part. This will maintain the moistness of the sample during transport. The bag shall be marked with the house identification, and the location of the bank and row from which the sample was taken. If egg collection is nonautomated, an additional manure sample from each bank, collected as described in clause (2)(i)(b) of this subdivision, shall be substituted as the second sample required from each bank.
(c) Selection of birds for organ culture.
Sixty birds shall be selected for organ culture in a random pattern throughout the house, with the exception that birds reacting to the blood test shall be cultured. Sample birds shall be sent to the diagnostic laboratory within two weeks after blood testing. A Salmonella-free environment shall be provided if the birds are held in cages overnight. All birds shall be identified by leg-band numbers. Birds from different houses shall be kept separated. The cage location from which birds originated shall be recorded. Organs shall be cultured according to the procedures outlined in section 57.9(b) of this Part.
1 CRR-NY 57.8
Current through July 31, 2023
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